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These engineered DNA-cutting enzymes make a precise break at specific locations in a cell's DNA, in this case in the gene that causes PNH.
If a precise break occurs, the ends are complementary, and therefore it can simply be reannealed back together and religated.
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Thus, those unmapped reads actually carry the information about the precise break points of the deletion event.
In this case we aim to compute the precise break points and the fragment inserted in the medium sized range (<=20 bases for 36 bp reads).
In Section 2.3, we explain how to compute the precise break points of deletion events and the deletion size could be rather large as long as we find unique matches for the two parts of the unmapped read.
In this case we know that 'ATGC' is the maximum unique substring of pattern P in this particular database S. In the Pindel program, we aim to compute the precise break points as well as the fragments inserted or deleted compared to the reference genome from paired-end reads.
The precise break-even point of Astroperations, has not been made public, but the stadium financial overhead is known to be Texas-sized.
This work, published Oct. 11 in Science, pinpointed the precise breaking point when the extreme pressures began to permanently deform the copper structure, or lattice, so it could no longer bounce back to its original shape.
This may be sufficient for those applications that do not need the precise breaking spots and that are tolerant to the transition errors described above.
Upon photo-illumination, ATLANS introduced a precise double-stranded break 12 bp downstream the TFO binding sequence and down-regulated the target gene in HeLa cell system.
Thus thermal resistance can be a precise indicator of fiber breaks and delamination in CFRP composites.
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