Therefore, the output of a preceding study supported us in defining the scope of the following study in more detail.
In a preceding study we have recently established an in vivo visualization system for the geranylgeranylation of proteins in a stably transformed tobacco BY-2 cell line, which involves expressing a dexamethasone-inducible GFP fused to the prenylable, carboxy-terminal basic domain of the rice calmodulin CaM61, which naturally bears a CaaL geranylgeranylation motif (GFP-BD-CVIL).
The accumulation and slower dissociation of 111In-Fab in tumours were in line with the slower off-rate of 111In-Fab compared to 125I-Fab observed on these tumour cells in vitro shown in a preceding study [31] using non-internalizing conditions, as discussed previously.
A culture isolated from ascospores of Hypoxylon rickii, a xylariaceous ascomycete collected in Martinique, had yielded botryane, noreremophilane and abietane-type terpenoids in a preceding study, but additional metabolites were detected by extensive HPLC MS analysis in other fractions.
2. PMH instrument (Stage 1): The self-administered PMH instrument used in Stage 1 consisted of 182 candidate items and was developed through focus group discussions with 65 respondents in the three ethnic groups in a preceding study where five domains of PMH were deemed relevant to this multi-ethnic population.
We initially selected gene families in the S. cerevisiae genome identified in a preceding study [ 31] that comprised only two members and synonymous divergence (K S) ≤ 0.35.
The mean IES-R subscale scores (avoidance, intrusion, hypervigilance) obtained in this study were compared with those in a preceding study [ 16].
In a preceding study of biomass allocation to aboveground plant organs, we did not record an increase in generative plant parts on unmown plots (Bartušková et al. 2015).
In a preceding study, indocyanine green (ICG) angiography was positively tested for detecting early stage flap malperfusion.
First, a non-linear variant of the Principal Component Analysis (Isomap) was performed in a preceding study to identify prevailing biogeochemical processes controlling water chemistry.
