High-throughput cDNA sequencing is a practical approach for detecting novel sequence variations and alterations in human tumors, such as those of the colon.
This rapid, simple, economical, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in Mycobacterium tuberculosis.
"I think it remains to be seen whether the approach will become a practical method for whole genome sequencing of these difficult but important ancient DNA samples, but I think it is exciting that this is even conceivable".
This study confirmed that whole genome sequencing is a practical approach for de novo assembly of higher plant mitochondrial genomes.
Our results confirmed that unenriched whole genome sequencing is a practical approach for de novo assembly of higher plant mitochondrial genomes.
These results confirmed that unenriched whole genome sequencing is a practical approach for de novo assembly of higher plant plastid and mitochondrial genomes.
In addition, we found that the use of a large dataset of almost 35 million reads permits the use of read abundance distributions along putative precursor sequences as a practical tool for isolating miRNAs in a large background of reads originating from other non-coding and coding RNAs.
Thus, the combined strategy of using a traditional approach to infer haplotypes using the genotypes at the common variants combined with assembly of the rare variants using the sequence reads is a practical approach for inferring haplotypes.
For a species that has neither its genome sequenced, nor a physical map available, the information obtained from expressed sequence tags (ESTs) is a practical means for gene discovery and a way to start elucidating its physiology and functional genome.
