These action potentials were applied as part of a sufficiently long train, and I f was computed according to the equations listed in Section 4. The resulting I f traces are shown in Figure 6(c), together with the net membrane current, Inet, which was computed from the time derivative (dV m / dt) of the membrane potential trace of Figure 6(a), as shown in Figure 6(b).
Most primer sets were designed to span an intron in order to avoid amplification from potential traces of genomic DNA in the total RNA preparations.
The use of this new set of local eQTLs allowed us to look for potential traces of selection where Col alleles were overexpressed in a set of genes with related function.
Na-DTPA (5 10 μL, 5 mM, pH 5) was added for complexation of potential traces of unreacted 152Tb and 177Lu, respectively.
More specifically, we first impaired a state-of-the-art SIFT-based copy-move forgery detector (briefly CMFD [3] by means of the CLBA attack[13]; then, we hid potential traces of keypoint removal using the aforementioned injection procedure.
Before in vivo application, the radioconjugate solution was diluted with phosphate buffered saline (PBS) (pH 7.4) and Na-DTPA (10 μL, 5 mM, pH 5) was added for the complexation of potential traces of unreacted 161Tb III) or 177Lu III).
Yet, potential traces of life (e.g. biogenically precipitated carbonates) may even be present in this ancient record (Nutman et al. 2010), suggesting that life itself can be several million years older than the oldest known stromatolites and microfossils.
Potential traces of endotoxin contamination were carefully removed from fucoidan fractions by the Detoxi-GelTM endotoxin removing gel (Pierce) and tested as described [12] using a Gel Clot Limulus Test raising levels lower than 0.5 IU/mg; Cape Code).
Samples were treated with DNAse I using the RNAse-free DNAse set (Qiagen) to remove potential traces of DNA in the sample; the absence of contaminating DNA was verified by failure to amplify the purified RNA samples prior to cDNA synthesis, using Taq polymerase and oligonucleotide primers targeting recA.
Importantly, no background fluorescence could be observed caused by potential traces of printing material.
