The described apical part of the alar ligaments is probably joined, in a growing potential manner, with the "proatlas center" and the lateral part depends on the "center 1" of the axis [ 4, 9].
Mitochondrial membrane Δψ was measured using the fluorescent lipophilic cationic dye tetramethylrhodamine methyl ester (TMRM), which accumulates within mitochondria in a potential dependent manner (62).
After 6, 12, or 24 h exposure to 4, 8, or 12 μg/ml of ZnO NPs, the cells were washed twice in PBS and were incubated for 30 min at 37°C with JC-1, a lipophilic and cationic dye that accumulates in the mitochondria in a potential-dependent manner.
JC-1 indicates mitochondrial polarization by shifting its fluorescence from green (FL1; ∼525 nm) to red (FL2; ∼590 nm) in a potential-sensitive manner due to concentration-dependent formation of red fluorescent J-aggregates.
This carbocyanine dye accumulates in the mitochondrial membrane in a potential-dependent manner.
JC-1 is a cationic fluorescent dye probe (green as monomer/red as aggregates) which accumulates in mitochondria in a potential-dependent manner.
JC-1 accumulates in mitochondria in a potential-dependent manner and emits fluorescence in the red spectrum (590 nm) indicating functionally active mitochondria (Smiley et al., 1991).
To further validate that the observed calcium flux is due to MPTP channel opening, we monitored the alterations in mitochondrial potential real-time by utilizing tetramethylrhodamine ethyl ester (TMRE) fluorophore, which reversibly diffuses into mitochondria in a potential-dependent manner.
Young and senescent NHEKs of the four subpopulations were stained with the JC-1 cationic dye, which accumulates in mitochondria in a potential-dependent manner and whose fluorescence shifts from red to green with mitochondrial depolarization.
Giuliani allowed that, as U.S. attorney for the Southern District of New York, he never would have turned over all of his investigatory material to a potential target in that manner.
