We also observed secondary spermatocyte nuclei still containing an H3K9me3 positive area, indicating that the GRC is not always eliminated at metaphase I (13 out of 180 nuclei; Fig. 3a).
Results are expressed as a percentage of positive area per total area of tissue.
We defined a bright green area as a YO-PRO-1 positive area (Figures 3B and 3D), and quantified YO-PRO-1 positive areas on rat and mouse retinas, comparing with those on acutely isolated retinas.
Noguchi et al. (2003) showed that the extent of metastatic skeletal lesions (percentage of positive area on a bone scan) is an independent predictor of death from disease in patients with prostate cancer.
Manchester United's defence has been one positive area in a difficult season for the club, but with Van Gaal appearing to be moving towards a more attacking mindset, there are even questions being asked of that department too.
We also found a correlation between the CD34 positive area fraction and C4d positive endothelial area fraction in diffusely infiltrating astrocytoma samples.
In addition, to evaluate the distribution pattern of the immunohistochemical staining intensity within the selected area, the fraction of immunohistochemically positive area at a desired intensity level was calculated.
Each core was given a score derived from the calculation of grade −1 + percentage/100 (e.g. 1.5 is the final score of a grade 2 with 50% positive area).
Several parameters per core were computed: the area of counterstaining, the ratio (as percentage) of the positive area vs counterstained areas and a quick score (percentage of positive area mean optical density (OD)).
