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Many loci showed a discontinuous allele distribution, with missing alleles across the allele size range, suggestive of a population contraction.
Allelic richness was higher in the historical samples compared to the contemporary samples, indicating a population contraction.
The significant positive values of SSD and raggedness and non-significant positive Fu's Fs (Table 4) also imply C. simplicipinna did not undergo a population expansion, so it maybe undergo a population contraction or stay a population dynamic equilibrium.
A star-burst distribution of haplotypes in the water rail lineages suggests a population contraction occurred, which would tend to erase less frequent haplotypes, and then the population expanded in size [ 27].
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A demographic history inferred from DNA sequencing data indicates that C. simplicipinna experienced a recent population contraction without retreating to a common refugium during the last glacial period.
Some genetic features of C. simplicipinna such as having high genetic differentiation among the populations, a clear genetic structure and a recent population contraction could provide guidelines for protecting this endangered species from extinction.
This study shows that this cycas species underwent a past population contraction during Pleistocene with high genetic differentiation among populations and a clear genetic structure.
The median value of the posterior distribution of the growth rate, g, was small and negative (around -2*10-5), i.e. compatible with a limited population contraction.
This expansion phase is followed by a period of population contraction resulting in only a small fraction of the expanded population surviving and entering the memory cell pool [1] [3].
In addition, to the paleontological record, genetic data from extant populations of collared lemmings in the Eurasian Arctic implies that this species underwent a continent-wide population contraction, possibly during one of the warm interglacials, followed by demographic expansion (e.g. [40], [47], [48]).
For other species that have a similar history of population contraction and expansion, the observations related to the lack of pattern of mtDNA over ESUs may provide an important lesson.
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