Because developing countries generally have a poorer context than developed countries, they confront many challenges in e-government implementation.
Changing the CUG context to a "Poor Kozak" context diminished the level of antigen presentation to almost that of the CCC[YL8] background.
However, there are exceptions: as a result of poor context (for example, noise), the first ATG could be ignored.
Compared to the strong toeprint band at +15 17 nucleotides with the AUG codon and a weaker band with the CUG codon in an "Excellent Kozak" context, a toeprint band was not detected when the CUG was in a "Poor Kozak" context (Fig. 2A, CUG Poor).
Furthermore, given the resource poor context, taking on a stewardship role might prove not to be beneficial as this could have negative financial implications.
Could it be possible that a general decrease in frequency of start codon recognition could just have a much greater effect on the weaker or poor context AUGs and relatively less on the AUG in a good sequence context?
However, even in the case of a gene with strong selection pressure (see Sharp et al. 45 for a discussion of selection pressure on codon usage), the first anticodon position will be a C in a GC-poor context.
