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The normalization in combination with a pooling of samples has the consequence that expression differences could not be reliably quantified from this data set.
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This paper proposes a new method to divide a pool of samples into calibration and validation subsets for multivariate modelling.
Standard curve obtained for the primer and PCR product from a pool of samples was used as template.
Here we present a novel strategy where the combination of two tags is used to link sequencing reads back to their origins from a pool of samples.
In wild boar, two pieces of the tonsils and a pool of samples from both mandibular lymph nodes were submitted for microbiology.
The PoolHap pipeline assumes that the investigators have sequenced a pool of samples and have run a mapping tool (e.g. BWA[11]) to map the short reads to a reference genome to call SNPs from the consensus sequence.
The software is also designed for the comparison between a sample (or a pool of samples) named 'A' and another sample (or a pool of samples) named 'B', each collected into specific tables.
A pool of samples from 4 patients with nonmalignant prostate inflammation was used as a reference sample.
Samples from each of four RA patients were run in the same gel with a pool of samples from four healthy individuals, designated the standard.
LD cDNA was diluted 1 in 4 and a pool of samples generated and serially diluted to generate a standard curve.
To select the subset of features, a pool of samples prepared from five different proliferating myoblast lines before and after myogenic differentiation were hybridized to two additional microarrays.
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CEO of Professional Science Editing for Scientists @ prosciediting.com