After 5 days, genomic DNA was isolated from the pool of surviving spores, and the allele frequency of each mutation was determined by methods described above.
DOI: http://dx.doi.org/10.7554/eLife.08467.004 For the MG132 resistance screen, we identified 992 independent insertion sites in the pool of surviving cells.
The numbers of surviving and newborn individuals are added and if this number exceeds the carrying capacity, we sample 10000 individuals out of the pool of surviving and new born individuals.
They travelled on foot across the Steppes – "all yellow grass and nothing else" – to Kazakhstan, at one point drinking from a pool of red water to survive.
It is conceivable that quiescence not only plays a pivotal role in the emergence of residual disease but also in creating a pool of tumor cells that survive continuous small molecule therapy and may hence represent the "seeds" for the outgrowth of resistant clones.
Then the floodgates opened, and Mr. Petrov was swept up in a wave of emotion as Russians seized on his Oscar as a victory for a culture, a tradition and a pool of artistic talent that has managed to survive despite the difficulties of the last decade.
Experiments have shown that once rats are made to feel helpless and then put into a pool of water, they give up trying to survive rather rapidly.
The high proportion of unclassified orthologous groups in the set of virulence-associated orthologs unique to V. cholerae represents a pool of unrecognized functionalities that may equip the species to survive and flourish in the environments it faces.
Given that intergenomic recombination and co-infection of individuals are common phenomena of MV it might be assumed that the lineages surviving till today did emerge from a pool of recombining pre-vaccination era strains.
Both survived as if they landed into a pool of silicon implants.
