To estimate efficiencies, a standard curve was generated for each primer pair from 10-fold serial dilutions (from 100 to 0.01 ng) of a pool of first-stranded cDNA template from all samples.
She spent half an hour on Friday afternoon lying in a pool of blood, first wondering if she had lost her husband and then fearing she would lose her leg, in Tunisia's worst ever terrorist attack.
Laura was this year's only Oxford applicant from a pool of 100 sixth-formers, according to the head, Dr Paul Kelley.
Barbic points out that students are selected in a lottery from a pool of mainly first-generation college-bound students, meaning it's not succeeding by skimming off the smartest students from the public system.
The level of expression of the M. persicae RyR gene was measured in different developmental stages of clone 4106A, including a mixed pool of first and second instars, mixed third and fourth instars and apterous and winged adults, but no statistically significant differences were found (Fig. 4).
Mars One intends to repeat this selection process on an annual basis, delivering six fresh teams of four every year, leading to a pool of 100 by first flight.
"Like an ink drop in a pool of water, at first you see the origin, but then it just dissipates and you can't see it," she said.
It was suggested that these CD4 lineage cells correspond to a pool of constitutively primed "first responder" cells [ 38].
Still, my grief over losing a wanted pregnancy, coupled with the terror of waking up in a pool of blood, was second only to my strong sense of guilt.
A total of nine hundred and sixty larvae from a pool of newly molted fifth instar larvae were evenly divided into eight groups according to their initial fresh weights (I).
