Sentence examples for a pool of constructs from inspiring English sources

This allowed the fast generation of a pool of constructs with different lengths of the repetitive sequences that can be easily recombined.

It can also be used for plasmid-based screens, recloning purposes, or to create a pool of constructs containing all or a subset of miRNA constructs for generating mixed lentiviral stocks.

To ensure that CL3EcoR cells were sufficiently sensitive to identify a single shRNAmir construct in a pool of 200 shRNAmir constructs, pRSp21F (a p21CIP1 shRNA construct) [ 17] was mixed in a ratio of 1 200 with pRSLaminA/C and used to assay bypass of senescence in CL3EcoR cells.

A pool of MLV-based constructs introduced into such cells will likely evolve over time and may spread to other cells, especially in co-culture experiments.

We transfected a pool of these four constructs into both NIH3T3 and Neuro2A cells along with pcDNA3.1/His/lacZ vector (Invitrogen) for G418 selection (Fig. 1A).

Next, we performed genome-wide shRNA screen in MCF-7 cells treated with RITA using a pool of lentiviral shRNA constructs, composed of 27 290 shRNAs targeting 5046 known human genes.

To rationalize the observed cytotoxic effects, structure-based virtual screening was carried out against a pool of potential targets constructed using the inverse docking program Tarfisdock and bibliographical references.

For each individual animal tissue sample, labeled complementary RNA (cRNA) was hybridized against a pool of labeled cRNAs constructed from equal aliquots of RNA for that specific tissue from at least 200 individuals.

In a series of initial calibration experiments we demonstrate how the barcode tiling approach can quantify the abundance of individual template molecules from a pool of 305 shRNA expression constructs.

After transfection of Neuro2A cells with a pool of the four siRNA constructs, we performed PCR-based cloning and DNA sequencing analyses with DNAs derived from each stable cell line to determine which construct(s) was the most potent in terms of macroH2A1 knockdown.

Neuronal PC12 cells were infected at DIV3 and at a multiplicity of infection (MOI) of 3. Primary neonatal rat SCG sympathetic neurons were infected at DIV 3 and at a MOI of 2, with lentiviral particles containing a pool of three different shRNAs constructs against rat parkin (shParkin) or a scrambled control sequence (shCT), both obtained from Santa Cruz Biotechnologies.