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For sensitivity testing, the target sequence was ligated into a plasmid vector and cloned.
We report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae.
We are constructing a plasmid vector that will allow insertion of any gene of interest at a defined site on the Bacillus anthracis chromosome.
This paper describes a plasmid vector for cloning cDNAs in Escherichia coli; the same vector also promotes expression of the cDNA segment in mammalian cells.
Here we developed a Salmonella typhimrium strain to maintain and safely deliver a plasmid vector to target tissues.
We prepared a plasmid vector coding a claudin-4 promoter-driven luciferase gene and established stable reporter gene-expressing cells.
The synthetic controls were amplified by PCR, cloned into a plasmid vector and transferred into bacteria host cells.
We report here the construction of a plasmid vector designed for the efficient electrotransformation of the periodontal pathogen Porphyromonas gingivalis.
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector.
Using a plasmid vector system to survey different inserted sequences for their dependence on introns for expression, we found that the presence of IVS2 stimulated the expression of these sequences 2- to 500-fold.
To this end we transfected tumor cells with a plasmid vector carrying a suicide cytosine deaminase gene driven by a promoter containing hypoxia response elements (HRE).
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CEO of Professional Science Editing for Scientists @ prosciediting.com