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Relative expression levels of the genes were calculated in relation to Gapdh expression (Applied Biosystems) using the ΔCt method with efficiency values measured in a pilot experiment for each expression assay.
The fact that amino acids encoded by RWY have simple lateral chains and were synthesized by Miller 31 in a pilot experiment for primordial earth conditions suggests that RWY is a relic of the prebiotic times at the origin of life.
ModENCODE was developed as pilot experiments using C. elegans [ 11] and D. melanogaster [ 12] to set up tools and to check for the feasibility of the ENCODE programme, much as the shot-gun D. melanogaster genome project [ 13] was a pilot experiment for the shot-gun human genome [ 14, 15].
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A pilot experiment testing for the production of PCR product over 4 5 orders of magnitude must be performed to optimize conditions for subsequent reactions.
A second reason for using reaching trajectories as our dependent measure came from a pilot experiment that revealed distinct MCEs for novel and repeated primes.
We discuss experimental parameters such as IR intensity, electrode design, and potential frequency for a pilot experiment to sort nanotubes with lengths ≈0.5μm.
We selected the colors for each experiment based on color-object association ratings obtained from 49 participants in a pilot experiment (see Additional file 1 for methodological details).
First, preliminary permission for a pilot experiment was approved.
We first performed a pilot experiment to treat the mice for 7 days to test whether JQ1 could downregulate ERα expression in vivo.
Highly contrasting results would suggest that every laboratory do a pilot experiment to identify genes suitable for use in normalization; similar results between the two systems would point to a set of genes reliable for broad application, minimally for the lines and developmental stages described.
Among other things, NWO is planning a pilot experiment to see whether additional training for reviewers can improve women's success rates.
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CEO of Professional Science Editing for Scientists @ prosciediting.com