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Candidates that are flagged red as "not likely" either failed to map in a pile of sequences in a very tight space of 15-25 nt of the predicted hairpin (e.g. were scattered evenly across the full length of the hairpin), mapped within the loop of the hairpin, or mapped to known tRNAs or rRNAs.
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As our technologies and understanding advance, will we eventually be able to look at a pile of raw DNA sequence and glean all the workings of the organism it belongs to?
Conditional probability distributions in this model were shown as follows: At each position, we observed a pile of bases oi with base qualities qi and a total sequencing depth di from the alignments of sequencing data.
It is common to discover a rhythmic pattern in a pile of stratified sedimentary rocks represented by a repetitive sequence of rock types.
Although a pile of dung from a cave poses serious analytical challenges, it's probably not the most difficult specimen researchers are sequencing now.
A pile of metal.
A pile of frosting, like a dream.
It is a pile of human beings.
"They killed a pile of us and we killed a pile of them".
That's quite a pile of rocks.
It's a pile of sticks".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com