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This was performed with a picture size of 512 × 512 pixels, 4 iterations and 16 subsets.
These sequences have a frame rate of 25 fps and a picture size of 352 × 288 pixels.
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List mode data, collected continuously over 60 min from the tracer injection, were reconstructed using Ordered Subset Estimation Maximum in 2 dimensions (OSEM2D) to increase the spatial resolution, with a picture size of 256 × 256 pixels, four iterations, and 16 subsets.
Optical sections were acquired at 1-µm intervals with a picture size of 512 × 512 pixels.
Optical sections taken at 1 µm intervals with a picture size of 512×512 pixels were processed using Adobe Photoshop CS3 and CS6.
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Optical sections range from 1 2 µm with a pictures size of 1024×1024 pixels.
For recovery of the fluorescence in TBs, images were recorded for 110 s, with a 512 × 512 px picture size, a scan speed of 167 ms/frame and a delay between frames of 0.2 s.
For controls, to account for fast tubulin diffusion in the cytosol, recovery was recorded in identical ROIs for only 5.9 s, with a 100 × 50 px picture size, a scan speed of 16 ms/frame and a delay between frames of 23 ms. We verified that the energy of the 488-nm laser used for the post-bleach added no bleaching effect by recording a control region outside the bleaching ROI.
The minimum throughput is a derived requirement from the picture size and minimum frame rate requirements.
But we've never had the torrent of emails we had in response to an error in Friday's paper when, in the graphic on page 2, we ran a picture the size of a postage stamp which we captioned as a character from the Gerry Anderson puppet show, Thunderbirds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com