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For the mtDNA analyses, a partitioned analysis was performed treating the cytb and cox2 genes as separate partitions, each with their own DNA substitution models.
A partitioned analysis was performed treating the cytb and 'Brown region' as separate partitions, each with their own DNA substitution models.
(1) Population stratification, which was attributable to co-occurrence of molecular forms (M and S), and cryptic within-form stratification necessitating both a partitioned analysis and genomic control.
A partitioned analysis (using the closest approximations in BEAST of the models applied in ML and BA, Table 3) never reached stationarity.
For the Cameroon data, despite the application of a huge value of λ (31.78) stratification could not be removed from the data by GC (Figure 3a) and to avoid both false positives and negatives (Figure 3c) a partitioned analysis of M and S was essential.
We assessed two possible methods of correction for stratification: a partitioned analysis (M and S analysed separately in Cameroon; and simply exclusion of M forms from Ghana owing to their rarity), and statistical correction of molecular form-related stratification by GC.
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They did not explore the phenomenon of expression dominance for the greater than 95% of genes that were not differentially expressed from the mid-parent, an exploration that requires a categorically partitioned analysis of the full set of genes (cf. Figures 4 and 5).
These charsets were then used in a partitioned Bayesian analysis, with each partition free to vary according to its own GTR + gamma model.
An additional positive aspect of adding process partitions to an analysis is that once a large number of partitions from various cellular functional classes are available, partitioned analysis will also allow detailed examination of the evolutionary dynamics of these classes of genes.
The second hypothesis predicts that a partitioned Bayesian analysis should support different phylogenies for pile-weave design characters as compared to the non-pile-weave design characters that include both flat-weave designs and weaving techniques.
We estimated divergence times using penalized likelihood (PL) in the program r8s v. 1.7 [45], [45], based on a Rag1 family-level phylogeny of salamanders with the topology and branch lengths estimated via a partitioned Bayesian analysis in MrBayes [41], [42] (Table S5).
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