Whereas CFP-Epac-YFP competes with the best FRET sensors in displaying robust FRET changes, we observed during extended characterization that this particular acceptor displays some reversible photochromism when excited with UV light (i.e., during cAMP uncaging experiments).
Interestingly that sialic acid is present at high concentrations in blood [34], thus it is possible that the immune system recognize TcTS in this particular acceptor-bound conformation.
The glycosyltransferases often transfer a specific carbohydrate from the appropriate sugar nucleotide (e.g., UDP-Glc, UDP-Gal, CMP-sialic acid) to a specific position on a particular type of acceptor (Cer or to the nonreducing end of the growing carbohydrate chain attached to Cer).
For each standard, there are measurable indicators, which range from the requirement that providers be licensed and registered (technical competence) to the prohibition of provider quotas for the number of family planning acceptors or acceptors of a particular method (assuring informed choice).
The exchange fluxes for a particular carbon source and electron acceptor were constrained to have lower bounds of −10 mmol/g AFDW/h (where g AFDW is grams ash free dry weight).
Some E2s directly mediate the formation of specific ubiquitin chains via the non-covalent binding of an acceptor ubiquitin, positioning a particular lysine residue to attack the thioester bond between the E2 and the donor ubiquitin (Petroski and Deshaies, 2005; Eddins et al, 2006; Wickliffe et al, 2011).
The acceptor arm binds to a particular amino acid, specified by the anticodon triplet located in the anticodon arm.
For a particular pair of carbon source and electron acceptor, growth phenotypes were qualitatively classified as growth if the model predicted growth rate is positive and no growth if the predicted growth rate is zero.
We found that under our culture conditions the predicted acceptor to donor ratio for a particular construct matched the ratio experimentally measured.
Sortases recognise not only a particular C-terminal sequence motif on the substrate protein but also a specific amino group on the acceptor to which transfer occurs.
