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To determine which of the two PPREs within the human NHE1 is the true PPARγ binding site, we transfected MCF-7 breast cancer cells with a full length NHE1 promoter construct (containing both PPRE1 and PPRE2) compared to a parallel set up transfected with a 5' deletion of the full length (only contains PPRE2).
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Others decided to stay in the Church of England, in a parallel set-up led by "flying bishops".
In addition, the fat separation was enhanced by reducing the transducer reflector distance in the vessel, operating two transducers in a parallel set-up, or by increasing the duration of insonation, resulting in skimmed milk with a fat concentration as low as 1.7% (w/v) using raw milk after 20 min insonation.
That parallel sets up some really meta moments in the premiere, like when Dan Conner John Goodmann) comforts his daughter Darlene (Sara Gilbert) saying, "[Roseanne] was going to do what she was going to do.
"This is a parallel track set up by enemies of Syria to compete with Kofi Annan's mission, maybe to undermine his mission".
As a parallel, Wissner-Gross set up a computer simulation in which there was a large disk, corresponding to the ape; a small disk caught in a tube, corresponding to the food; and another small disk near the large disk, corresponding to the tool.
Of course, all strategies need to be considered in light of the alternative minimum tax, a parallel tax system set up in 1969 to ensure that the wealthiest taxpayers paid their fair share of taxes.
Aid agencies also want a parallel agreement to set up registers of who owns and controls anonymous "shell companies" to be made public rather than limiting access to them to tax authorities.
The AIF was administered separately from the home-based army in Australia, and a parallel system was set up to deal with non-operational matters including record-keeping, finance, ordnance, personnel, quartermaster and other issues.
A parallel reaction was set up for every experiment with the inhibitor of respective complexes to assure the specificity of the reaction.
To measure Notch activity from the co-culture, a parallel assay was set up as above, with the following modification: MDA-MB-231 cells were co-transfected with 12xCSL-Luc (30 μg/dish) and MH100-HSP-lacZ (3.0 μg/dish).
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