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As expected, catalytic activity of Cel5A in mutated constructs was reduced to 191.1 U/mg and to 536.1 U/mg, respectively, while the substrate affinity was only slightly affected, proving a parallel release of reducing sugar ends by Bgl1 in double-active fusion enzymes (Table 2).
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The reduced amount of released Cr VI) analysed in solution is most likely a result of the parallel release of acids and/or reducing agents from the leather (43).
This was in agreement with the accumulation of LTB4 at the site of inflammation in vivo and the parallel release of LTB4 from neutrophils but not macrophages upon immune complex (IC -induced IC -inducedtination in our own hands.
There is a possibility that the measured amounts of released Cr VI) in the extraction solution are underestimated when the leather sample is ground or cut into small pieces as compared with whole leather surfaces, owing to the parallel release of reducing species from the leather.
Fast vesicle release, which is triggered by calcium influx through one or two calcium channels [ 49], tight coupling of these channels to the vesicle-release sites [ 49], and parallel release of multiple vesicles through the ribbons [ 50], all ensure high rate and temporal precision of release of the neurotransmitter glutamate.
We previously showed that in primary mouse cortical neurons exposed to OGD apoptosis precedes necrosis, as indicated by the early TUNEL-positivity displayed by the cells within 6 h after the OGD and the parallel release of cytochrome c in the cytosol in absence of lactate dehydrogenase (LDH) release.
We observed a time-dependent decrease in intracellular retinyl ester and a parallel extracellular release of retinol when pHSCs were preincubated with retinol and palmitate and then chased in retinol-free medium.
The immediate goal is for the parallel release to help start a potential new movie franchise.
Correction: August 26 , 2004 Thursday Because of an editing error, an article on July 15 about an official British report that criticized the country's prewar intelligence on Iraq misstated one finding of a parallel report released the previous week by the United States Senate Intelligence Committee.
In parallel, the release of IL-6 looked to be delayed compared to that of IL-1β and TNF-α (Fig. 3b, c, d).
Interestingly, the improved killing activity parallels an increased release of specific cytokines, whose production is altered during HIV infection.
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