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To study the effect of alteramide A on P. citrinum, a solution of 10 μg of alteramide A in MeOH was applied to a paper disk (6 mm diameter), air-dried, and placed at a 5 mm distance from a 1 μL inoculum of P. citrinum on M1 agar in a Petri dish.
Make a paper disk.
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After ψt was measured, branches were removed from the chambers, sap expressed to saturate a filter paper disk with a Markhart Leaf Press and osmotic potential measured with a C-52 chamber.
The seeds were centrifuged in an Eppendorf centrifuge for 1 min, the supernatant removed, and the seeds were resuspended in 1 ml of 100%% ethanol and immediately pipetted onto a filter paper disk in a sterile hood.
In this test, the antimicrobial is placed on a filter paper disk which is incubated on a plate which has been streaked to grow a lawn of bacteria.
A chromatography paper disk (diameter 2.0 mm) cut by a laser cutter (Epilog Zing 16, Golden, CO) was placed inside each LAMP zone, as a 3D storage substrate for LAMP primers.
The tissue suspension was collected pouring 5 ml aliquots onto a filter paper disk (80 g/m2 43 48 μm; Filter Lab, ANOIA; Barcelona, Spain) in a Büchner funnel.
The reaction mixture was spotted onto a P81 paper disk (Whatman), washed in 15% phosphoric acid, and counted in a liquid scintillation counter (Beckman Coulter).
Uniform corneal epithelial defects were created in the right eyes with the application of a filter paper disk soaked in 75% ethanol solution in the center of the tissue for 60 seconds.
(2) 10 µl CFS was dropped onto a 6-mm paper disk on an agar plate spread with cell suspension of a given indicator strain, and incubated 24 h at 30 °C.
Eight microliters of the diluted drug were added to a 6 mm paper disk on a sheep blood agar plate.
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