We therefore selected a panel of 17 tumour sections (nine were highly positive, three with medium to low staining and five with low to negative staining), which we analysed by polymerase chain reaction-single-strand conformation polymorphism analysis (PCR-SSCP) for the presence of a p53 mutation at the molecular level.
This was investigated by immune-staining a panel of frozen tissue sections from Rag-1 −/− mice (to prevent background staining by endogenous immunoglobulins) with sera from WT control, TKO or DKO chimeras, and visualized using confocal microscopy (n=5 reconstituted mice per genotype).
Immunohistochemical analysis of SOX9 protein expression was conducted in a panel of 42 NF1 tumour sections, 10 of which were independent of the gene expression microarray experiment (Fig 3E).
A3 and CC7 were then tested by immunofluorescence for their ability to detect CA IX in a panel of xenografted human tumour tissue sections.
The cutoff values for losses and gains were defined using a panel of 10 paraffin-embedded control sections of non-neoplastic tissues and were calculated as 10%% for gain and 12 % for loss.
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Sandwiched between two spacious, open internal sections are a panel of five red neoprene pouches.
Immunofluorescence results of total and phospho-ERK1/2 on a panel of 19 fresh frozen uveal melanoma sections are listed in Table 2 (B).
Immunohistochemistry was carried out on 4 μm thick sections for a panel of potential molecular membrane bound targets known to be expressed in a frequency of >10% in breast cancer.
To overcome these limitations, we developed a triple immunostaining technique, which is applicable to both cytology slides and paraffin sections, using a panel of markers consisting of p27, Galectin-3, and HBME-1.
