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For the single sequence with no heterogeneous sites, the HBV genotypes were determined by phylogenetic analysis with a panel of reference sequences from genotypes A to H retrieved from GenBank.
The method was validated using a panel of reference DNA from the University of California, Los Angeles, International DNA Exchange Program.
The calculated Tissue Preferential Expression (TPE) value is based both on the presence of a particular tags and its level of expression in the SAGE library of interest in comparison to a panel of reference SAGE libraries derived from a range of different whole tissues.
Viruses were propagated in 10-day old embryonated hens' eggs and initial subtype identification was performed using standard hemagglutination-inhibition (HI) assays and neuraminidase inhibition assays [21] using a panel of reference antisera and reference antigens obtained from the OIE Avian Influenza Reference Laboratory (VLA Weybridge-UK).
The reference genes used were selected from a panel of reference genes by applying a gene stability algorithm [ 20].
The housekeeper genes used were selected from a panel of reference genes (RG) according to the GeNorm algorithm[ 28].
Housekeeper genes were selected from a panel of reference genes (RG) according to the GeNorm algorithm and represent the best reference genes for canine mammary gland.
GAPDH was used as a reference gene for the multi-organ panel, while H3F3A was previously found to be the most stably expressed from a panel of reference genes tested in the mammary gland samples [ 46] as determined using Genorm [ 53].
Credits on each item are derived from the answers given by a panel of reference.
Credits on each item are derived from the answers supplied by a panel of reference.
The normalization for target genes expression was used as an example to illustrate the essentiality of a suitable reference gene selected from a panel of candidate reference genes.
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