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Among all the synthesized compounds, we identified a panel of active inhibitors with Ki values towards one or two chymotrypsin-like activities of proteasome (β5c) and immunoproteasome (β5i and β1i subunits) in the low micromolar range.
Indeed, by employing the evolved 202 08 intein, a panel of active scFv- and GFP-intein fusion proteins could be displayed or secreted from yeast and directly functionalized via EPL without any solubilization or refolding steps.
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However, the tested samples were less active than the RA on all of the microbial strains, this observation been quite normal as it is expected that a crude extract (that contained a panel of both active and non active molecules) shows less activity than a purified compound.
A panel of 28 active kinases was selected from the hit list, based on their activity and classes, and silenced by their corresponding siRNAs in four breast cancer cell lines, MDA-MB-231, SUM149, BT474-M1, and HR5.
To this end, we performed an in vitro screen using recombinant YAP and a panel of recombinant, active kinases.
Collectively, these data suggest that MSCs secrete a panel of functionally active factors that act in the healing of the injured tissue microenvironment.
A panel of selected active kinases was then further tested on four different breast cancer cell lines to confirm the spectrum of growth inhibition.
Since 1994 the Institute for Studies of the Medical Profession at the Norwegian Medical Association has regularly surveyed a representative panel of active Norwegian doctors with mailed questionnaires.
The empirical study on a panel of European R&D active companies shows that both R&D intensity and patents increase with the degree of technological diversification of the firm.
(Lauraceae) and Entada gigas (Linn) Fawcelt & Rendle (Mimosaceae) against a panel of MDR Gram-negative bacteria expressing active efflux pumps.
We have previously reported on fever screening at airports in Taiwan as part of active surveillance for a panel of notifiable infectious diseases such as dengue, gastroenteritis caused by enteric bacteria, malaria, and yellow fever (6 ).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com