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The only major requirement is a pair of unambiguous amplification primers that will work for the particular group of interest.
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Males of Phasmatodea possess a sclerotised hook on the venter of abdominal segment 10, the vomer, between a pair of unsegmented cerci, which are interpreted as unambiguous apomorphic characters for Phasmatodea [ 58], both exhibited by Renphasma [ 56].
A pair of dolphins.
A pair of shoes?
A pair of friends.
A pair of platform shoes.
A pair of tens arrived.
A pair of boots 26.
A pair of defective marriages.
A pair of Levi's.
Imagine a pair of electrons.
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