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A pair of tissue samples for each case was collected from the tumor tissues and their corresponding noncancerous colon tissues.
The abstraction involves a pair of tissue specific dynamical system models of the same biological process, both manipulated synchronously through a set of common input functions.
The cumulus-oocyte mass was released from the ampulla of the oviduct by rupturing the oviduct with a pair of tissue forceps into 0.1% bovine testicular hyaluronidase (Sigma-Aldrich) in M2 medium (Specialty Media, Phillipsburg, NJ) to disperse the cumulus cells.
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The corresponding hybridizations with a ZmTCRR-1 probe are shown for comparison; these results show that the transfer cells express a pair of tissue-specific, developmentally controlled response regulators.
When applied to actual mouse RNA-Seq datasets from six tissues, IUTA identified 2,073 significant genes with clear patterns of differential isoform usage between a pair of tissues.
The number of genes that were differentially expressed between a pair of tissues in tissue set one was used as a distance metric, and hierarchical clustering, using complete linkage (44), was applied to this distance matrix to cluster tissues.
Most such studies have independently mapped eQTLs in two or more tissues and contrasted the number of eQTLs found in both tissues, arriving at estimates of the overlap between a pair of tissues ranging from 12 to 80%%.
A low value of VI between a pair of tissues indicates a high degree of correlation in APA regulation, whereas a high value of VI indicates a weak correlation.
The strength of connection between a pair of tissues with regard to gene expression is defined as, where i and j correspond to probesets in tissue 1 and tissue 2, and N 1 and N 2 are the number of probesets in tissue 1 and tissue 2, respectively.
Genes which were significantly different in any pair of tissue types were kept for further analysis.
For each heart, a pair of adjacent tissue sections was prepared.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com