FOXO1-AAA-ER was expressed from pLPCX retroviral vector (Clontech, Inc .. Recombinant retroviral stocks were produced by co-transfecting 293T cells with an appropriate vector and a packaging construct (pCL10A1 from Imgenex, Inc).
The Time Warner Center was cleared after a "suspicious package" constructed with a pipe and wires was discovered in CNN's mailroom.
The consensus phylogenetic tree of the NCLDVs was obtained from a tree constructed from a concatenated alignment of four universal NCVOGs [6]: primase-helicase (NCVOG0023), DNA polymerase (NCVOG0038), packaging ATPase (NCVOG0249), and A2L-like transcription factor (NCVOG0262).
So he's a cyborg constructed from scrap kitchen equipment?
The dnadist program of the PHYLIP package 3.68 [82] was used to calculate nucleotide distances using the F84 model and a tree was constructed from those distances using the neighbor-joining method [83], as implemented in PHYLIP's neighbor program [82].
A profile HMM was constructed from the alignment by using the program HMMER software package [ 48], and used for gene prediction.
The sequences alignments were performed using CLUSTAL_X [ 19], and a phylogenetic tree was constructed from evolutionary distances using the neighbor-joining method of MEGA 5 program package [ 20].
A profile HMM was constructed from the alignment of known OR sequences from human, frog, and fish using the HMMER software package (http://hmmer.janelia.org).org
This work designs and evaluates a stream-based mail proxy constructed from several open-source packages.
A calibration curve was constructed from individual ant measurements.
FRAIG is a package for constructing AIGs for Boolean functions while enforcing functional uniqueness of each AIG node.
