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We performed a control experiment with a pH 9 aqueous solution of NaOH (Supplementary Note 4).
In order to mimic the acidic lysosome lumen, we entrapped inside the supported membrane an acidic solution (pH 5), while in the extravesicular milieu we maintained a neutral pH solution (pH 7.4) reflecting the cytoplasmic pH.
The combined solution was then mixed with a pH 5.0 citric acid solution to produce a final pH of 5.5.
Look for a neutral pH detergents solution.
Pure water, which is neutral, has a pH of 7. A solution with a pH less than 7 is considered acidic, and a solution with a pH greater than 7 is considered basic, or alkaline.
Then, we use the proposed PIF algorithm as a key module in designing snap-stabilizing solutions for some fundamental problems in distributed systems, such as Leader Election, Reset, Snapshot, and Termination Detection.
Liposomes were loaded with either an acidic (pH 5.0) or a neutral (pH 7.4) buffer solution.
Developing self-stabilizing solutions is considered to be more challenging and complicated than developing classical solutions, where a proper initialization of the variables can be assumed.
Instead, clean concrete with a pH-neutral cleaner like a solution of liquid dish soap and water, working up to harsher solutions as needed.
This would usually result in a solution with a pH ≈ 6.5.
Immersion in a mild acidic solution, with a pH of 5.5, worked best.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com