B. subtilis was transformed with the constructs yielding strains with promoter-lacZ or -gfp fusions inserted in the amyE locus using a one-step transformation method [40].
Therefore, the recent advent of HV generator (Powerformer) that can be connected directly to the power transmission line has motivated the design of HV transformer (Dryformer) that performs one step transformation from transmission to distribution voltage levels.
The synthesis of 3,4-diaryl-5-unsubstituted isoxazoles 13 was designed based on a condensation of arylbenzaldehydes, arylnitromethanes, and ethoxycarbonylmethylpyridinium bromide followed by a selective one-step transformation of intermediate 3,4-diaryl-5-ethoxycarbonyl-4,5-dihydroisoxazole 2-oxides 8.
The actual transformation of plasmid recipient is readily achieved by a one step treatment of the Vac- SD109 strain with spheroplasting solution.
Prodrugs 6 8, are synthesised in a high yielding one-step transformation from the active parents with high diastereomeric excess.
Herein, novel sandwich-like Na0.23TiO2/Ti3C2 composites made of 1D amorphous Na0.23TiO2 nanobelts growing on 2D Ti3C2 nanosheets have been prepared through a one-step scalable transformation reaction of Ti3C2 MXene.
Here we describe a novel system-level methodology for unbiased cloning of intact large SM clusters from a single fungal genome for one-step transformation and expression in a model host.
Based on these observations we propose a simplification of previously designed one-step transformation procedures for B. subtilis strain 168.
For overexpression of Rub1, pRD54-HA-Rub1 or pRD54-HARub1ΔGG was transformed into S. cerevisiae S288c using one-step transformation, and expression was induced by addition of 2% final concentration galactose.
The one-step transformation concept is adopted for easy programming.
As the correspondence obtained by the closest point searching method for the random points is not exactly the true correspondence according to skull and face feature, instead of making one-step transformation from the reference to target, we do the TPS transformation in a stepwise procedure.
