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On the basal medium containing the optimized concentration of metal ions, the effects of four main raw materials on the spore yields conducted in a one-factor experiment are presented in Table 3.
To verify the validity of this complexity measure, a one-factor experiment was designed to test the proposed hypotheses.
The experimental design utilized in the present study was on the basis of one factor experiment at a time.
The significant metal ions chosen from the one-factor experiment, Mn2+ (x1), Fe2+ (x2), Ca2+ (x3) and Mg2+ (x4) were included in the CCD for the determination of their optimum concentrations, and the results are listed in Table 2.
Based on the results of one-factor experiments, four parameters were further optimized by an orthogonal test (L 9)(3)(4)).
Two one-factor experiments were designed on a simulated RVD system.
The induction conditions were systematically examined by combining one-factor experiments with an orthogonal test (L 9)(3)(4)).
Several one-factor experiments and an orthogonal test (L 9)(3)(4)) were employed to optimize the induction conditions.
The suitable induction conditions were systematically optimized by combining one-factor experiments with an orthogonal experiment (L 9)(3)(4)).
To improve the expression level of IFN-CSP, the induction conditions [ 16] like cultivation temperature, induction timing, inducer concentrations, induction time were systematically examined by combining one-factor experiments with an orthogonal test (L 9)(3)(4)).
It has been shown elsewhere, for simple one-factor experiments, that statistical testing can be conducted directly on those summary statistic trajectories using Random Field Theory RFTT).
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