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We performed western blot analysis for the BCR-ABL1 fusion protein using mouse monoclonal antibody against c-Abl [c-Abl(24-11); Santa CA]z, Santa Cruz, CA] or GAPDH (6C1, Millipore, Billerica, MA) and IR Dye800CW-labelled anti-mouse IgG (Li-cor, Lincoln, NE) and detected the proteins using a Odyssey scanner (Li-cor).
Proteins were visualized with IRdye-coupled secondary antibodies on a Odyssey scanner followed by analysis with Odyssey software (LI-COR Biosciences).
Blots were incubated with ECL substrate (GE Healthcare, Pollards Wood, UK), scanned with a Odyssey Imager (LI-COR) and analyzed with Image Studio software (LI-COR, version 1.1.7) using background correction.
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A band corresponding to each protein was visualized using an Odyssey scanner and Odyssey 2.1 software (Li-Cor, Lincoln, NE).
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