A striatal nucleus, area X, is necessary for song development and vocalisation, and is only present in the male brain.
Along with this we also noticed a significant increase in nucleus area after 72 hours and 4000 ng/mL DON although not in a dose-dependent way.
It is noteworthy that we detected a significant enlargement in nucleus area for 4000 ng/mL DON from basolateral for 72 hours incubation only (Tab. 1).
In addition, a general increase in cell nucleus area was evident.
We noticed also a significant increase of the cell number per area (10,000 μm) and a significant decrease of the nucleus area in both cell lines.
TO-PRO-3 images were used to make a mask that removed the nucleus area and staining from the E-cadherin.
In apical and sub-apical cells from caulonemata, the tip or tip-oriented area and the nucleus area display a significantly higher amount of chloroplasts than the base area (See Additional file 2).
The precise temporal regulation of FoxP2 that occurs only during singing, and the regional restriction of this regulation to song control nucleus area X strongly suggests that FoxP2 has a post-organizational role in learned vocalizations.
There is a significantly higher density in the tip area versus the nucleus area and in the nucleus area versus the base of the apical cells, and in the tip-oriented area versus the base of the sub-apical cells (See Additional file 2).
In contrast, other types of cells were completely opposite, with a shorter optical path difference in the cytoplasm area rather than the nucleus area.
Irregularities in nuclei shape do not affect how the algorithm calculates average cell nucleus area, as it does not constrain expressed fluorescent area to a specific shape.
