Sentence examples for a nuclei from inspiring English sources

A nuclei isolation kit (Nuclei EZ Prep Kit, Sigma-Aldrich Chemie Gmbh, Steinheim, Germany) was used to separate and quantify the amount of radioactivity stored in the nuclei [28].

Their nuclei were prepared using a nuclei extraction kit NE-PER (Pierce), resuspended in 2 mL PBS containing 1 mM EDTA, 200 μg/mL RNase A, and 50 μg/mL propidium iodide, and then analyzed on a FACS Calibur platform (Becton Dickinson) using an excitation wavelength of 488 nm.

This triple heterokaryon could have arisen from an interstitial crossover; that in N. crassa would have produced a 6 2 ascus with two each of the T a, N A, Dp a, and Df A nuclei.

In addition, a nuclei filter was used to remove over-segmented nuclei.

Environmental sequences were translated into proteins, and the previous 309,245 sequences were BLASTed all against all (BLASTP, e-value threshold = 1e-5, 5000 hits to avoid artefactual increases in the distance between environmental ORFs and nuclei in similarity networks, in case a given sequence from a nuclei hits > 500 environmental ORFs).

Nuclei were isolated from mop1 1 mutants and WT immature ears using a nuclei isolation buffer modified from Steinmüller and Apel, 1986.

r right|} right.}}} ;dr{prime}Here summation runs over all nuclei A in a molecule, polarization and reorganization effects are ignored.

In contrast, propidium iodide (PI), a nuclei-staining dye, can only pass through the disordered areas of the dead cell membrane and intercalates with the DNA double helix of the cell to emit a red fluorescence (excitation: 535 nm, emission: 617 nm).

To examine the surface barrier function, living worms were incubated for 15 min in buffer containing a nuclei-staining dye Hoechst 33342, which does not usually permeate the worm cuticle barrier.

The lack of such changes in whole-cell lysates or the supernatant and pellet fractions of sonicated samples confirmed the incomplete lysis hypothesis and indicated that LY30 had no effect on Hsp27 expression but instead induced its translocation to a nuclei-enriched pellet fraction.

This enabled us to construct two general types of heterokaryons with mat-A and mat-a nuclei of different genotypes: one type is [ T + N] (with one translocation nucleus and one normal sequence nucleus), and the other is [ Dp + Df] (with one nucleus carrying a duplication of the translocation region and the other being deleted for the translocation region).