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Cell cycle analysis by flow cytometry showed that fun30-deletion mutants do not exhibit a noticeable defect in cell cycle progression (Figure 3 B, wt, fun30Δ).
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Unlike in ctf18Δ cells, we could not observe any noticeable defect in sister chromatid cohesion in fen1Δ and dna2-2 cells.
Initial studies strongly suggested that occludin was not required for the TJs formation or the maintenance of barrier function as occludin knockout mice lacked any noticeable defect in intestinal TJs morphology or barrier function [ 36].
Both the hot embossing and UV nanoimprint patterning processes were done on large size substrates without any noticeable defects.
While this appears to be an excellent hypothesis, knockdown of the DEPDC2 gene along with CPA6 in the zebrafish, using specific splice-blocking morpholinos, did not result in any noticeable defects in eye abduction (data not shown).
Combined loss of BOK and BAK did not elicit any noticeable defects, although it remains possible that BOK and BAK have critical roles in developmental cell death that overlap with those of BAX.
Indeed, although overproduction of UBP10 caused a noticeable growth defect in wildtype cells, this defect did not worsen when combined with the rsp5-1 mutatinn, in striking contrast to UBP2.
Of the 17 mutants with no obvious growth impairment at 25°, 11 displayed a noticeable growth defect when animals were shifted to 30°.
There were no wing hair defects (orientation or number) or any other noticeable defect associated with the loss of dGIPC either in homozygous animals (even when the maternal contribution was removed), or in homozygous somatic clones.
The first noticeable defect was observed at telophase I when lagging chromosomes were observed, followed by mis-segregated chromosomes at prophase II.
The first noticeable defect was the appearance of lagging and broken chromosomes during telophase I. Twenty or more individual chromosomes were often observed beginning at metaphase II, suggesting that cohesion might be prematurely lost.
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