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Method 3 is a normalized procedure, performed under well controlled conditions and therefore enables the surface state to evolve from one state to another at well controllable rates.
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A normalization procedure should be applied to obtain a normalized combination (8) w i = w i ∑ j w j.
Selected arrays were normalized according to the GC-RMA normalization procedure [ 14].
After each array was normalized within array, the arrays were then normalized across arrays using the A-quantile normalization procedure.
The quantification of the target genes was conducted using a calibrator-normalized procedure with the alpha-tubulin (atub) gene [GenBank: AJ279695] of silver birch and the putative PP2A [ 76, 77] of silver birch as reference genes.
Each array was then normalized using loess normalization and then across arrays using the A-quantile normalization procedure [27].
We also consciously used non-normalized RNA samples as the downstream BSA requires quantitative estimates of frequency ratios, which would be perturbed by a normalization procedure.
We then developed a simple normalization procedure to process the data and allow the generation of a normalized, highly contrasted, chromosomal contact map for S. cerevisiae.
This work provides guidance on testing and evaluating detection devices, and may contribute to the establishment of suitable standards and normalized procedures.
We will present both static bubble rise and dynamic (kill) data using the normalized procedures and develop simple predictive formulas for interpolation purposes.
Based on a study of 25 different types of materials, the normalized undrained shear strength of clay shales was found to correlate satisfactorily with overconsolidation ratio following the SHANSEP (Stress History and Normalized Soil Engineering Properties) normalization procedure for clays.
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