The phrase "a normalisation factor based on" is correct and usable in written English.
It can be used in contexts involving statistical analysis, data processing, or mathematical calculations where a factor is adjusted according to certain criteria or data points.
Example: "To ensure accurate results, we applied a normalisation factor based on the average values of the dataset."
Alternatives: "a scaling factor derived from" or "a standardisation factor determined by".
Calculating a combination normalisation factor based on the geometric mean of three genes for the kairomone experiment and for the growth experiment, stressor-specific regulation of some of the genes involved in basic metabolism is demonstrated.
One involves the use of a normalisation factor (NF) based on the geometric mean of multiple carefully selected reference genes.
A normalisation factor, calculated based on the geometric mean of the three reference genes, was used to normalise the expression of each gene of interest.
A normalisation factor was calculated based on the endogenous controls assessed by geNorm [ 52] according to Schwarzenberger et al., 2009 [ 53].
We use the geNorm algorithm [ 10] to estimate the variability of the reference genes, and to discover an optimal normalisation factor (NF), based on the geometric mean of three of them.
In order to determine how many reference genes should be included, normalisation factors (NFn), based on the geometric mean of the expression levels of the n best reference genes, were calculated by inclusion of an extra, less stable, reference gene according to Vandesompele et al [ 9].
In addition, we applied a normalisation protocol based on combinations of reference genes determined by geNorm (Vandesompele et al, 2002), as developed for HCC (Supplementary Figure 1).
In [ 18] they propose using a normalisation factor calculated as the geometric mean of the relative quantity (RQ) values of the reference genes, based on previous work [ 7, 17].
The denominator on the right hand side represents a normalisation factor accounting for the average net income and population size.
After determination of a normalisation factor from the set of reference genes, the raw data of the QPCR runs were imported into qBase version 1.3.5 [ 37], an Excel (Microsoft) based tool which calculates relative gene expression normalised using the normalisation factor generated with geNorm.
