To verify whether the difference in fluorescence growing patterns could be attributed to the differential soluble expression of the fusions, cells were analyzed for their expression in native form in soluble portion and non-native one in aggregation by SDS-PAGE.
Which ultimately led to research comparing the native fire ants with the non-native ones, and an interest in how the imported fire ant gets along in Brazil.
A typical approach is to develop a knowledge-based scoring function to discriminate native structures from non-native ones and to sample this potential in search of the global minimum [4].
Then in order to enhance the performance of the prediction method, we optimize the linear parameters of the energy function, so that the native-like conformations become energetically more favorable than the non-native ones for proteins with known structures.
According to Gaugler (1988 , 1997 the native EPNs provides superior control when compared to non-native ones.
Do native species have a chance to come inside a forest rich in exotic species when available habitats might have been occupied by those non-native ones?
European freshwater mussels (Fig. 1) are threatened by the presence of non-native ones, principally zebra mussel and secondarily Chinese pond mussel (Cappelletti et al. 2009).
Finally, it would be interesting to investigate to which extent our analysis could contribute to reliable discriminatory functions that can distinguish near-native conformations from non-native ones.
In our earlier studies, we had exploited protein structure networks to discriminate the native structures from the non-native ones.
The scheme herein only produced a weak bias to the reference structure since native non-bonded interactions can be broken and new non-native ones formed, in contrast to Folding-based models where non-native contacts are unfavorable.
In our previous studies, we have discussed the importance of the transition profile of the various network parameters as a function of I min to characterize the native structures and therefore distinguish them from the non-native ones.
