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For example, one base-calling error can result in a no alignment match to the reference genome, a match reporting a false SNP, or a misaligned match to a different region of the genome.
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Being different from the traditional CoMFA, topomerCoMFA is a No-Alignment CoMFA [21].
In cases where this procedure does not produce an MCS, no alignment is performed.
ESTScan was used to decide the sequence direction and to predict coding regions of a unigene if no alignment resulted from the above databases [ 65].
If a unigene had no alignment hit (match) in the above databases, ESTScan software [ 16] was used to predict the CDS domain and its direction.
Two countries had neither a research agenda nor alignment (Chile, Korea), and one country reported a research agenda but no alignment (Kazakstan).
This is as expected for a composite material with no alignment of fibers.
In addition, since SOAP2 and Bowtie do not have alignment quality scores in their respective output files, to ensure a relatively fair comparison, no alignment quality filters are used.
This process is repeated until no alignment with a higher score is found.
0° indicates perfect alignment with respect to the flow direction while a value of 45° indicates no alignment.
Bold and italic, both interacting residues are present; italic, only one residue is present; dash, no alignment possible a Analysis was performed using Clustal W. b each combination represents an allele in yeast mutagenesis studies.
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