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For our application, constructive future work in a next cycle could include extending to regional level and accounting for temporal variability.
The amount remaining may serve as an innoculum for a next cycle of growth.
Thereafter, a next cycle of denaturation, annealing, elongation and cleaving is started again, resulting in exponential amplification of the duplex oligonucleotide.
To start a next cycle, ANC had to be ⩾1.0 × 10 l−1, platelets ⩾75 × 10 l−1, AST, ALT and serum bilirubin ⩽4 × ULN, and patients had to have no other toxicity of common toxicity criteria (CTC) Grade 3 or 4 with the exception of nausea, vomiting and anaemia.
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In most cases, this will mean that it is in the patient's best interest to have a delay in the next cycle of chemotherapy to allow time for a full recovery, and then a modification of the dose or regimen to reduce the risk of further neutropenic fever/sepsis.
According to the rules established by successive randomised trials, it is a current practice to postpone the next cycle for a week and/or to use lower drug doses below an accepted neutrophil count of 1.5 × 10 l−10
If a haematology test within 72 h before the next cycle indicated a neutrophil count <1500 per mm or a platelet count <100 000 per mm, the next cycle was delayed until haematologic recovery.
It is a possibility in the next cycle, maybe in six years.
The causes for deviations are however hard to identify by visual inspection and complicate the correction of a problem in the next cycle or batch.
Also, it assists in estimating the bandwidth needed by a SS in the next cycle.
But the threats will remain, and come to haunt us again with a vengeance when the next cycle begins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com