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The medium in the cultures was changed every 7 days, and a new SSc PBMC culture supernatant or medium control (each 30% by volume) was added at each refeeding.
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However, further studies are needed to establish this locus firmly as a new susceptibility SSc genetic risk factor.
The present study identified NF-κB and PLCL2 as new SSc susceptibility genes and confirmed the IRF8 locus.
Dr. Xiaoxiao Zuo's group [9] conducted an in-depth review specifically focused on the epigenetic regulation on SSc and pointed out the promising direction for new SSc biomarkers.
Remarkably, two of the four new SSc genetic-susceptibility markers identified in the previously mentioned study might play a relevant role in the SSc-related fibrotic process, SOX5 and NOTCH4 [ 4].
Moreover, miR-196a levels in hair shafts are stably decreased in SSc patients, indicating that this miRNA can be used as a new biomarker for SSc diagnosis [29].
At our institution, we employ a new model for SSc management that combines "in person" appointments and "remote" therapy (SMART).
The aldose-1-epimerase thus represents a new candidate for SSC variation.
On top of that, ADAM12 levels predicted the improvement in thrombospondin levels and might be a new biomarker for SSc.
We have described the SSC, a new tool for exact stochastic simulations of biochemical reaction networks.
Using a combination of markers for the known epidermal cell types, new markers and ultrastructural analyses we have characterised a new cell type, the SSCs, in the Xenopus larval epidermis.
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