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Cells from the same population that were not exposed to BrdU were used as a negative staining control to determine background-staining levels for the anti-BrdU monoclonal antibody.
The representative images show different staining intensities of GNA13: (a) negative staining, (b) weak staining, (c) moderate staining, and (d) strong staining (original magnification, x100 and x400).
Uranyl acetate was used as a negative staining agent.
Assembly conditions duplicated those of a recent light scattering study and specimens were prepared at different time points with the use of a negative staining technique recently employed to visualize the trinodular structure of fibrinogen.
To verify HRP in the hollow spheres, a negative staining sample was stirred in 1% aqueous uranyl acetate (UA) for 1 h, and then centrifuged to remove the remaining UA.
A negative staining was classified as having an immunostaining score of 0 3; whereas a positive staining was classified as having an immunostaining score of 4 12 (at least moderately positive in at least 11%50%% of cells).
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A negative stain reconstruction was used filtered to ~60 Å resolution for the starting model.
It's a negative stain on the culture of football if we have to implement a Rooney rule.
A negative stain EM structure of the TtCsm complex exhibits the characteristic architecture of Type I and Type III CRISPR-associated ribonucleoprotein complexes.
Our study presents the first 3D of the full-length human LRRK1 dimer structure and provides additional structural details to the recent report of the LRRK2 dimer structure based on a negative stain EM28.
(A ) Negative stain EM map of HAP-ClpP.
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