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Interestingly, both isolated clones exhibited high selectivity for H1299 cells without using a negative selection step.
Clones that underwent targeted exchange are selected solely based on loss of a negative selection marker at the target site.
At this point a negative selection procedure would be possible by using ganciclovir to select clones that have lost the tk fusion protein by site-specific cassette exchange.
Splice-site targeting outperformed a conventional CRISPR library in a negative selection screen targeting 79 ribosomal genes.
Potentially considering CH hypersensibility as a negative selection aimed goal in donkey breeding programs, may turn into a measure to improve animal welfare indirectly.
A negative selection approach is thus preferred for tumour cell isolation as it does not depend on biomarker expression or defines their physical property as the separation criteria.
The two resulting detectors undergo a negative selection but are not split again.
Second, there is a negative selection effect: new participants are less productive (conditioning on human capital) than existing participants.
There might have been a negative selection bias, in which more patients with persistent complaints returned the questionnaire.
Despite the prevalence of mosaic aneuploidy, hESCs derived from IVF blastocysts are mostly karyotypically normal, suggesting that aneuploidy may act as a negative selection barrier for hESC derivation.
For example, there could be a negative selection process driven by motivation or other unobservable factors such that individuals with unfavorable characteristics are sanctioned with higher probability.
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