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11 Finally, the morbidity associated with completion dissection is significantly greater than that of SLND for melanoma, so that the clinical cost of a negative procedure is greater than with SLND alone.
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Even though the acid treated consignments were not allowed to enter feed mill until a negative test procedure, the decontamination process cannot be considered as a guarantee for freedom from salmonella but instead that the level of contamination has been brought under the detection level of the test method applied.
These obscure orders give effect to ministerial decisions and simply come into force within 40 days unless revoked (in either House) by "negative procedure" – an almost unheard of occurrence.
The enrichment of NK cells was based on a negative selection procedure of peripheral blood mononuclear cells (PBMNCs) using Miltenyi microbeads coupled to anti-CD3 (T cell depletion) and anti-CD19 (B cell depletion) mAbs.
This was felt by the adolescents to be a negative discriminative procedure.
T lymphocytes were isolated from peripheral blood (PB) and synovial fluid through a negative isolation procedure (Dynal).
Staining with an irrelevant IgG or an isotype-specific antibody was routinely performed as a negative control procedure in the normal tissues and in all the clinical samples.
At this point a negative selection procedure would be possible by using ganciclovir to select clones that have lost the tk fusion protein by site-specific cassette exchange.
In 10 patients with metastatic breast disease, a negative selection procedure was performed for the isolation of CTCs according to Naume et al [ 23].
A negative depletion procedure was used to enrich for CTC, combining gates containing CD45 negative and CD34 negative cells in addition to 7-amino-actinomycin D viability staining to exclude all haematological and non-viable (G fraction) cells.
To test whether ROS generation by PBL was due to T cells, we performed a negative selection procedure specific for T cell isolation in 12 controls and in 23 SSc samples and ROS production was analyzed.
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