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Although specific lipids in bacteria can be quantitated, there is still a need to quantitate the total lipid content of a bacterial sample.
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The need to quantitate the penetration of antibiotic-like molecules into bacterial cells remains a major barrier to this understanding.
In this chapter, focus has been placed on tracer experiment design strategies needed to quantitate production and utilisation fluxes of a substance under both steady- and nonsteady-state conditions.
Additional studies are needed to quantitate the presumably greater mortality rates that result from default and drug resistance in other settings.
Unfortunately, publications in this area primarily address single, relatively small teams of less than 10 20 people that do not interact with other teams as part of a larger working unit [23] We therefore needed a general way to quantitate efficiency as a function of group size in the context of multiple project teams.
We developed a software to quantitate the difference in expression.
Blots were scanned by a densitometer to quantitate differences.
Blots were scanned by a densitometer to quantitate differences, and a parallel gel was silver stained to ensure equal loading.
The mouse β-actin primers were used as a control to quantitate the fold change in PDI gene expression.
Blots were scanned by a densitometer to quantitate the differences and analysed using 'Phoretix ID Advanced' software.
The HERmark assay still demonstrated a greater ability to quantitate HER2 expression over a wider dynamic range.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com