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The deviation of the line from zero revealed a need for normalization, so prior to data analyses we normalized signals using a locally weighted scatterplot-smoothing regression (LOWESS) algorithm implemented in BASE.
Thus, there is a need for normalization and analysis methods that reduce false positives in the HTS experiments (Dragiev et al., 2012, Kwan and Birmingham, 2010, Murie et al., 2013, Rieber et al., 2009, Seiler et al., 2008).
Similar(58)
Just as with high-dimensional gene expression microarray platforms [ 32, 36, 34, 38, 37], the bias curves observed here demonstrate that bias is not constant across all abundance levels for high-dimensional microRNA data indicating a need for nonlinear normalization.
Overall, these data show good technical reproducibility within SAMs and a need for non-linear normalization.
However, the length dependence of muscle activation and purported, substantial, muscle translocation and changes in muscle architecture during dynamic movements presents a need for joint angle-dependent normalization processes.
This approach allows for an unbiased comparison among individuals with a wide range of hematocrit without the potential need for normalization, and also eliminates the impact of incidental dilution with other bodily fluids (e.g., lymph) during collection of the blood sample.
An upgrade in the software used for base calling after completion of the second biological replicate resulted in an increase in the overall number of reads collected, emphasizing the need for normalization across runs.
The FSM captures the signal of differential transcription directly from a pair of ACT-Graphs, without knowledge or inference of the underlying transcripts or need for normalization.
The need for normalization, when dealing with samples from different sources, was already shown for microarray-based high throughput technology [25].
Additionally, the density distribution of the two labels Cy3 and Cy5 were examined by applying limmas plot densities function [73] and the differing density distributions indicate the need for normalization.
b i, which describes the need for normalization, was set to be zero.
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