The phrase "a necessary restriction" is correct and usable in written English.
It can be used when discussing limitations or constraints that are essential for a particular purpose or outcome.
Example: "In order to ensure safety, a necessary restriction on the use of heavy machinery has been implemented."
Alternatives: "an essential limitation" or "a vital constraint".
In part this was a necessary restriction because if we opened up the 19th century it could easily have become a module about imperialism and its victims.
Condition (H5) is imposed for simplicity and estimates of solutions [29], p.60, and we will see it is not a necessary restriction of (3); in the case we will consider (see Corollary 4.6 below).
The wide range of incubation periods seen cannot be readily interpreted due to the very small numbers of animals in this study (a necessary restriction due to the very limited amount of suitable donor material, and limited number of recipients of a suitable genotype).
These DNA sequences were delivered in the plasmid pMA amp) and pCR4Blunt-TOPO amp,kan) containing all necessary restriction sites, His6-tag, GS-linker and cleavable enterokinase site.
In addition, in the case of any necessary restrictions on the process of randomization (i.e. block randomization [ 2]) the complexity of the process will be increased even further and be prone to errors.
Nonetheless, previously described "bad words" in MPSS sequencing reactions [ 25], as well as the absence of the necessary restriction sites within a particular cDNA, dilution of transcripts with low expression in diverse transcriptomes, and the cost-prohibitive nature of replicates are shortcomings of the technology.
But the necessary restriction of cars as part of the Walthamstow scheme has angered local businesses.
It consists of decreased amount of carbohydrate intake with no necessary restriction of energy intake.
The age-1 mg44), age-1 mg44) andaf-16 m27u86) daf-16 m27re tranded using single worm PCR and, where necessallelesstriction digests.
The open reading frame was amplified with primers 5′ NotI and 3′ BamH I to add the necessary restriction sites for cloning into the pS91-IRES-NEO vector.
If necessary restriction sites can be generated using the Quickchange site-directed mutagenesis kit (Stratagene), taking care to exclude the possibility that the mutations affect promoter function with appropriate transfection assays.
