The phrase "a native affinity" is correct and usable in written English.
It can be used to describe a natural liking or attraction that someone has towards something, often in the context of culture, language, or environment.
Example: "Her ability to learn languages quickly is a testament to her native affinity for communication."
Alternatives: "an inherent attraction" or "a natural inclination".
Overall, our results indicate that RNAs ranging in size from 246 341 nt, ending with a 3' overhang of one nucleotide, and purified using the native affinity technique, could be successfully used in a wide range of biochemical and structural assays.
This observation was confirmed by the allosteric properties of 1 3 hybrid tetramers to which only a single allosteric ligand and a single substrate ligand were able to bind with native affinity.
We also applied the standard native affinity purification method to 12 sequence variants of a 287 nt long group I intron from the bacterium Clostridium botulinum.
Here, based on X-ray crystal structure analysis and calorimetric data, we designed and synthesized photoreleasable biotins, which show greatly reduced affinity for (strept avidin, but recover native affinity after UV irradiation.
These shortcomings were addressed in the next generation of this native affinity purification technique [8].
Native affinity purification was performed with strains P19-Tbx1-PA and P19-PA as previously described [35].
These results demonstrate the suitability of the native affinity purification method for the preparation of group I introns.
Here, we report the PCR-based construction from overlapping primers of six group I intron genes belonging to four different organisms, as well as the native affinity purification of the corresponding RNAs (246 341 nt) using the first-generation method.
Similarly, GST-EWS-FLI1 could not be taken further for native affinity purification because of its insolubility.
