Design intermediates have levels of epitope modification between wild-type and germline-targeting trimers; their mutation gradient suggests sequential immunization to induce bnAbs, in which the germline-targeting prime is followed by progressively less-mutated design intermediates and, lastly, with native trimers.
In this study we designed a mutated form of hEGF (mEGF) with a deletion of four amino acids residues (Gln43, Tyr44, Arg45 and Asp46) in order to show importance of Leu spatial location for EGFR binding/activation.
Hence, using the cre-loxP system, we designed a mutated allele of Grp94 that allows both the traditional knockout and conditional knockout in a tissue or time specific manner.
A mutated form of EPO was designed by changing amino acid 46 from V to D and amino acid 50 from A to E, and the construct was cloned into the pBI vector.
We also designed an experimental procedure using a mutated GFP gene containing a frameshift mutation that results in a loss of GFP expression.
A mutated ε-toxin gene lacking toxicity was designed, synthesized, and cloned into the pT1NX vector and electroporated into Lactobacillus casei competent cells to yield LC-pT1NX-ε recombinant strain.
LCR primers, including a biotin-labeled common primer and two fluorescence-labeled allele-specific primers, are designed for two alleles of a mutated site.
Here, we conjugate a phosphorodiamidate morpholino oligomer with a designed cell-penetrating peptide (PPMO) targeting a mutated dystrophin exon.
This design would hint at the idea of selection of a mutated pyruvate kinase protein specific for ATP cofactor.
BONUS: Check out Hertzfeld's design for Frox, a home computer that looks more like a mutated Wii than anything available back in 1990.
Methods: To examine the role of ATDC in pancreatic cancer, we designed an anti-ATDC small inhibitory RNA (siRNA) as well as a mutated anti-ATDC siRNA (msiRNA) to serve as a control.
