These plasmids were used to generate triple R-to-A mutants of SLP65 (R49, 248 and 313A), or the SLP76* variant accommodating the CIN85 binding sites (K85P, L334P, M337R and N404R) by site-directed mutagenesis (Quikchange).
It is well known that the amylose-extender (ae) mutant of rice, that is deficient in BEIIb gene, exhibited a severe chalky phenotype of grain.
Although the inhabitants of Pringles had never seen a mutant of this kind, they were mainly country folk, familiar with the principles of procreation.
In familial cardiac amyloidosis, the amyloid fibrils consist of prealbumin, a mutant of transthyretin.
For this purpose, we have chosen to study a mutant of DT, cross-reacting material 107 (CRM107).
This confirms that S012 is a mutant of S001, and that it withstands high glycerol concentration more than the wild.
The purified protein was identified by peptide mass fingerprinting matching the record of gi|2624856, a mutant of DsbA.
The comparison of specific activity may facilitate recognizing a mutant of small improvement of activity, but is prevented from practicing by the lack of efficient methods for selective assay of a mutant protein in its lysate.
We constructed over-expression (OX) lines and RNA-interference (Ri) lines, and selected a mutant of OsNPF7.2, a low-affinity nitrate transporter.
This vector harbors a codon-optimized gene encoding a mutant of phosphite dehydrogenase (PTDH) with an N-terminal His-tag (Torres Pazmiño 2009b).
A mutant of C. tropicalis American Type Culture Collection (ATCC) 20962 that was isolated via direct evolution using a continuous culture was used in this study.
