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Interestingly, the SA content was in a much lower basal level in the mutant than in Wangshuibai, implying that the mutant have SA synthesis deficiency.
In gamergates, which have a much lower basal catalase activity, the reduction was only slight but also highly significant (statistics, see above, t = 8.37, p<0.001).
In contrast, CLE209 was only induced about 5-fold over a much lower basal expression level relative to CLE210 such that CLE210 mRNA was ~37-fold more abundant than CLE209 at day 2 but only ~6-fold more abundant at day 10.
Thus, the different lipid composition of the ER that results in a much lower basal cholesterol activation threshold would also be expected to amplify the oxysterol-induced membrane effects, allowing cholesterol-homeostatic machinery to be more sensitive to small changes in cellular cholesterol levels.
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By engineering two copies of the tet operators flanking the TATA box of the human U6 promoter, we created a U6 promoter derivative (2O2) that exhibited much lower basal transcriptional activity compared with recently reported inducible pol III dependent promoters.
Only the probe sets for Lbx1 and its corepressor, Lbxcor1, showed significant signal reductions in the white cell comparison, albeit from much lower basal signals than in the green cell comparison.
When equal numbers of live bax +/+ and bax -/- cells were seeded and mock-treated, bax -/- cells produced more colonies, consistent with the much lower basal level of cell death in these cultures (see Figure 4).
Following treatment with LY294002, a dose-dependent reduction in BCL-2 promoter activity was observed only in the MIA-PaCa-2 cell line, but not in the PANC-1 cell line, which has a much lower level of basal AKT activation.
Post-treatment basal expression of mIL1R2 in steroid responders was detected at a much lower cycle number by Q-RT-PCR than pre-treatment (figure 3B).
measured at a much lower level.
It was a much lower number in 2010.
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